Cloning, characterization and expression of chalcone synthase from medicinal plant Rhus chinensis

Authors Ma, Wenli; Wu, Yang; Wu, Min; Ren, Zhumei; Zhong, Yang

Journal JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY , ,:

Abstract Rhus chinensis is a well-known Chinese medicinal plant and chalcone synthase (CHS) is a key enzyme involved in flavonoid biosynthesis. In this paper, both the cDNA and the genomic sequence encoding CHS from R. chinensis were cloned and anaylzed. The ORF of RcCHS was 1,173 bp in size and predicted to encode a 390-amino acid polypeptide. Phylogenetic analysis showed that RcCHS was grouped into a branch with legume plants Glycine max and Medicago sativa. Real-time quantitative PCR showed that RcCHS gene expression were clearly tissue-specific, but not correlated with total anthocyanins accumulation as previously reported in some other plants. Moreover RcCHS was highly expressed in E.coli with pGEX-6P-1 vector, which will be helpful for further research on the structure, cellular localization and catalytic mechanisms of RcCHS.

Species covered 紫苜蓿 Medicago sativa 大豆 Glycine max 盐肤木(变种) Rhus chinensis