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The effects of Glycyrrhizae uralenis and its major bioactive components on pharmacokinetics of daphnetin in Cortex daphnes in rats

作者 Zhang, Wen; Di, Liu-qing; Li, Jun-Song; Shan, Jin-jun; Kang, An; Qian, Shuai; Chen, Le-tian

期刊 JOURNAL OF ETHNOPHARMACOLOGY , MAR 25 2021,:268

DOI 10.1016/j.jep.2020.113556 PubMed 33157223

摘要 Ethnopharmacological relevance: Glycyrrhizae uralenis (GU) is often prescribed together with Cortex daphnes (CD) in traditional Chinese medicinal practice to increase the efficacy of CD on the treatment of rheumatoid arthritis (RA), but the reasons were still unknown. In order to clarify the rationality of herbaceous compatibility between CD and GU, the comparative evaluations on pharmacokinetic behaviors of daphnetin (a predominantly active ingredient in CD) after intragastric administration of CD and CD-GU (combination of CD and GU) extract were studied. In addition, the effects of glycyrrhizin and liquiritin, active ingredients of Glycyrrhiza triterpenes and Glycyrrhiza flavones respectively, on the pharmacokinetics of daphnetin were also investigated.Materials and methods: Five groups of rats were orally administered with CD extract, CD-GU extract, pure daphnetin, co-administration of daphnetin and glycyrrhizin as well as co-administration of daphnetin and liquiritin at the same single dose of daphnetin (20 mg/kg). The rat plasma concentrations of daphnetin were determined by our developed UPLC-MS/MS method. The pharmacokinetics of daphnetin in above groups were investigated and compared.Results: Comparing with oral administration of CD extract, AUC and T-max of daphnetin significantly increased after giving CD-GU (p<0.05). In addition, in comparison to daphnetin alone, co-administration of daphnetin with liquiritin significantly increased the AUC and T-max of daphnetin for similar to 1.5-fold, while co-administered with glycyrrhizin showed limited impact on the pharmacokinetics of daphnetin.Conclusions: In this study, it was found that liquiritin, one of the major components of GU, significantly enhanced the bioavailability of the main component daphnetin in CD. In addition, the bioavailability of daphnetin in the CD-GU prescription was also significantly higher than that in CD alone, which could be due to liquiritin. Such results explained the mechanism of the increased efficacy in treating RA with the combined use of CD and GU. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 甘草属 Glycyrrhiza