Isolation of beta-sitosterol from Hibiscus sabdariffa L.

Authors Piyatida, P.; Kimura, F.; Sato, M.; Kato-Noguchi, H.

Journal ALLELOPATHY JOURNAL , OCT 2013,2:32

Abstract The aqueous methanol extracts of H. sabdariffa inhibited the growth of 12-test plant species, cress (Lepidum sativum L.), lettuce (Lactuca sativa L.), alfalfa (Medicago sativa L.), timothy (Phleum pratense L.), Italian ryegrass (Lolium multiflorum Lam.), ryegrass (Lolium rigidum Gaud.), crabgrass (Digitaria sanguinalis L.), buckwheat (Eriogonum compositum Douglas ex Benth.), Chinese sprangletop (Leptochloa chinensis [L.] Nees.), jungle rice (Echinochloa colona [L.] Link.), barnyardgrass (Echinochloa crus-galli [L.] Beauv.) and sand fescue (Festuca myuros L.) in a dose-dependent manner. The aqueous methanol extract of H. sabdariffa was purified and an active substance was isolated and identified as beta-sitosterol by H-1- and C-13-NMR analysis. The threshold of beta-sitosterol for growth inhibition was 0.3 mu M. The I-50 values on cress and lettuce were 16.2 and 406.7 mu M, respectively, whereas these values were 10.2 and 61.0 mu M on timothy and Italian ryegrass. The endogenous concentration of beta-sitosterol in H. sabdariffa was 32.7 mu mol/kg.

Species covered 紫苜蓿 Medicago sativa 莴苣 Lactuca sativa 马唐 Digitaria sanguinalis 千金子 Leptochloa chinensis 玫瑰茄 Hibiscus sabdariffa