Nitrite scavenging effect of alcohol extracts of thirty-eight medicinal plants

Authors Zhao Yan; Leng Yantao; Yu Ting; Gao Yugang; Zhang Yue; Zhang Lianxue; Xi Guangsheng

Journal Journal of Northwest A & F University. Natural Science Edition , 2017,2:45

Abstract 【Objective】 This study screened medicinal plants with nitrite scavenging effect and investigated the correlation between NO_2~- scavenging rate and total phenolic content of extracts to provide basis for exploitation of medicinal plant resources in Linjiang.【Method】 According to sulfanilic acid diazotization of nitrite under acidic condition, coupling compound was formed through reaction with hydrochloric acid naphthyl ethylenediamine. WD-2102A automatic microplate reader was used to determine the absorbance of solution, nitrite clearance was calculated, and samples with high clearance rate were further determined to calculate IC_(50). Simple linear regression analysis was used to analyze the correlation between NO_2~- scavenging rate and contents of total phenols in the extracts. 【Result】 The ethanol extracts from 38 medicinal plants had different NO_2~- scavenging effects. The scavenging rates were positively related to the contents of total phenols following equation Y = 0.915 5x+17.644 with correlation coefficient R2 of 0.323. The 9 species with NO_2~- scavenging rate greater than 55% (including different parts of same plant) were further measured. The NO_2~- removal rate increased with the increase of concentrations of alcohol extracts. The N02 scavenging abilities of plants were in the decreasing order of Lycopus parviflorus Maxim.>Rubia cordifolia Linn. >Vitis vinifera (seed)>Geum aleppicum Jacq. >Vc>Acer ginnala Maxim. (branch)> Agrimonia pilosa Ledeb. >Oenothera erythrosepala Borb. > Acer ginnala Maxim. (leaf) Acer ginnala Maxim. (fruit) > Artemisia stolonifera (Maxim.) Komar > Xanthium italicum Moretti (fruit).【Conclusion】 Vitis vinifera (seed) and Acer ginnala Maxim. had the most promising potential for development of NO_2~- scavenger among the 38 species in Linjiang and polyphenolic constituents were the main active substance for NO_2~- scavenging.

Species covered 茜草 Rubia cordifolia 路边青 Geum aleppicum 龙芽草 Agrimonia pilosa 黄花月见草 Oenothera glazioviana 茶条槭 Acer ginnala