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Melanin-concentrating hormone-1 receptor antagonism and anti-obesity effects of ethanolic extract from Morus alba leaves in diet-induced obese mice

作者 Oh, Kwang-Seok; Ryu, Shi Yong; Lee, Sunghou; Seo, Ho Won; Oh, Byung Koo; Kim, Young Sup; Lee, Byung Ho

期刊 JOURNAL OF ETHNOPHARMACOLOGY , MAR 18 2009,2:122

DOI 10.1016/j.jep.2009.01.020 PubMed 19330910

摘要 Ethnopharmacological relevance: In Korea, Morus alba leaves have been traditionally administered as natural therapeutic agent for the alleviating dropsy and diabetes.Aim of the study: The present study was performed to evaluate melanin-concentrating hormone receptor subtype 1 (MCH1) antagonism of the ethanol extract of Morus alba leaves (EMA) and its anti-obesity effect in diet-induced obese (DIO) mice.Materials and methods: The binding affinity of EMA for the MCH1 receptor with europium-labeled MCH (Eu-MCH), the function of recombinant MCH1 receptors expressed in CHO cells, and the anti-obesity effects in DIO mice were evaluated.Results: MCH1 receptor binding studies showed, EMA exhibited a potent inhibitory activity with IC50 value of 2.3 +/- 1.0 mu g/ml. EMA (10-100 mu g/ml) also inhibited the intracellular calcium mobilization with the recombinant MCH1 receptors expressed in CHO cells. In an anti-obesity study with DIO mice, long-term oral administrations of EMA for 32 consecutive days produced a dose-dependent decrease in body weight and hepatic lipid accumulation.Conclusions: These results suggest that chronic treatment with EMA exerts an anti-obesity effect in DIO mice, and its direct MCH1 receptor antagonism may contribute to decrease body weight. Crown Copyright (C) 2009 Published by Elsevier Ireland Ltd. All rights reserved.

涉及物种 桑 Morus alba