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WIDE-SCALE POLLEN BANKING OF ORNAMENTAL PLANTS THROUGH CRYOPRESERVATION

Authors Xu, Jin; Li, Ling; Liu, Qian; Shi, Yin; Peng, Jingen; Jia, Mengxue; Liu, Yan

Journal CRYOLETTERS , JUL-AUG 2014,4:35

PubMed 25282499

Abstract BACKGROUND: Cryopreservation has been proved to be an efficient method for the long-term storage of pollen, based on our previous studies establishing cryo pollen banks for Camellia, Paeonia, and Prunus mume. OBJECTIVE: In the present study, we aimed to extend and verify the applicability of the cryopreservation protocol for ornamental plants. MATERIALS AND METHODS: 71 species/cultivars from 19 families were cryopreserved using the established protocol. RESULTS: Pollen from 60 species/cultivars (85% of those experimented on) retained viability after 1 year''s cryopreservation; of these, 36 (51% of the total investigated) retained a sufficiently high germination capability compared with fresh pollen to enable longer term cryopreservation. CONCLUSION: This study confirms that wide-scale pollen banking of ornamental plants is feasible.

Species covered 芍药属 Paeonia