Study on Soil Enzyme Activities in GAP Base of Chinese Medicinal Plant of Gentiana Scabra Bge.

Authors Qu Bao; Wang Dongmei; Si Lianfei; Ge Caiyan; Tan Xiaomei; Pan Yingni; Liu Xiaoqiu

Journal Chinese Journal of Soil Science , 2016,6:47

Abstract The methods of sodium thiosulfate titration, permanganate titration, TTC- dehydrogenase determination and phenyl phosphate disodium salt colorimetry, respectively, were used to determine the activities of soil invertase, catalase, dehydrogenase and phosphatases in Good Agricultural Practices (GAP) base of Gentiana scabra Bge. The enzyme activities were studied in rhizosphere and bulk soil during different growing periods of gentian. Results showed that the higher activities of soil catalase and dehydrogenase, respectively, appeared in June and July. The activities of soil invertase were higher in July or August. The activities of phosphatases increased with time and reached the maximum value in September. The maximum value of total enzyme activity index appeared in August. The activities of soil catalase and dehydrogenase improved, and those of phosphatases and invertase had no obvious changes with the cultivation years. In sum, soil enzyme activity showed different seasonal variation with different cultivation years. The activities of invertase and catalase were lower and those of dehydrogenase and phosphatases were higher in bulk soil compared with in rhizosphere soil. The results of correlation analysis showed that there was a significant negative correlation between catalase and acid or neutral phosphatase activities, and positive correlation between the activities of acidic, neutral and alkaline phosphatase. Also the significant positive correlation was found between the total enzyme activity index and the activities of acid phosphatase, neutral phosphatase or invertase. This research completed the information of soil enzyme activities in the gentian GAP base, and provided scientific basis for comprehensive evaluation of soil fertility and reasonable application of fertilizer to improve quality of gentian.

Species covered 龙胆 Gentiana scabra