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Inhibitory activities of some traditional Chinese herbs against testosterone 5 alpha-reductase and effects of Cacumen platycladi on hair re-growth in testosterone-treated mice

作者 Zhang, Bei; Zhang, Rong-weng; Yin, Xi-quan; Lao, Zi-zhao; Zhang, Zhe; Wu, Qing-guang; Yu, Liang-wen; Lai, Xiao-ping; Wan, Yu-hua; Li, Geng

期刊 JOURNAL OF ETHNOPHARMACOLOGY , JAN 11 2016,:177

DOI 10.1016/j.jep.2015.11.012 PubMed 26571086

摘要 Ethnopharmacological relevance: Many traditional Chinese medicines (TCM) have been used for hundreds of years for hair blackening and hair nourishing, and now many of them are commonly used in Chinese herbal shampoo to nourish the hair and promote hair growth.Aims of the study: The present study was performed to screen 5 alpha-reductase (5 alpha R) inhibitors from traditional Chinese medicines, evaluate its hair growth promoting activity in vivo, and further investigate its effects on androgen metabolism and the expression of 5 alpha R II in hair follicles.Materials and methods: Nine TCM which were dried, ground and extracted by maceration with 75% ethanol or distilled water were used for screening 5 alpha R inhibitors, and enzymes were extracted from the rat epididymis. The leaves of Platycladus orientalis (L.) Franco was used to evaluate the in vivo antiandrogenic activity. Skin color was observed daily and the hair re-growth was assessed by assigning the hair growth score. The longitudinal sections of hair follicles were used for observing follicle morphology, classifying of distinct stages of hair follicle morphogenesis and calculate the average score. The transverse sections were used for determination of hair follicle counts. Testosterone (T), Dihydrotestosterone (DHT) and Estradiol (E2) levels in serum and skin tissue were detected by ELISA kits. The immunofluorescence assay was used to detect the influence of CP-ext on 5 alpha R expression in dorsal skin.Results: We found the extract of Ganoderma lucidum (GL-ext), Polygonum multiflori (PM-ext), Cacumen platycladi (CP-ext) and Cynomorium songaricum (CS-ext) showed stronger 5 alpha R inhibitory activity. CP-ext (5 mg and 2 mg/mouse/day) could significantly shorten the time of the dorsal skin darkening and got longhaired (P < 0.01), and showed high hair re-growth promoting activity. Furthermore the histological data of hair follicles in each group showed that CP-ext could promote the growth of hair follicle and slowed down hair follicles enter the telogen. What''s more CP-ext significantly reduced DHT levels and down-regulated the expression of 5 alpha Rllin skin (P < 0.01).Conclusions: GL-ext, PM-ext, CP-ext and CS-ext showed strong 5aR inhibitory activity. CP-ext possesses high hair growth promoting activity in the in vivo androgen-sensitive mouse model via inhibiting the 5 alpha R activity, decreasing the DHT levels and in turn suppressing the expression of 5 alpha R. Our study may contribute to the development of a new generation of herbal supplements with clearer material basis of pharmacodynamic for treating androgenic alopecia (AGA). (c) 2015 Elsevier Ireland Ltd. All rights reserved.

涉及物种 侧柏 Platycladus orientalis 蓼属 Polygonum 锁阳 Cynomorium songaricum