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Lignans from the stems of Clematis armandii (Chuan-Mu-Tong) and their anti-neuroinflammatory activities

作者 Xiong, Juan; Van-Binh Bui; Liu, Xin-Hua; Hong, Zhi-Lai; Yang, Guo-Xun; Hu, Jin-Feng

期刊 JOURNAL OF ETHNOPHARMACOLOGY , MAY 14 2014,3:153

DOI 10.1016/j.jep.2014.03.036 PubMed 24661966

摘要 Ethnopharmacological relevance: The dried stems of Clematis armandii (Caulis clematidis armandii), named Chuan-Mu-Tong in Chinese Pharmacopoeia, have been traditionally used as an herbal remedy mainly for inflammation-associated diseases. The Aim of the study is to identify the potential anti-neuroinflammatory components from Clematis armandii.Materials and methods: The ethanol extract of Chuan-Mu-Tong was suspended in H2O and exhaustively extracted with CH2Cl2. The CH2Cl2 fraction was successively subjected to column chromatography (CC) over silica gel, Sephadex LH-20, and semi-preparative HPLC. The structures of the isolated compounds were identified by spectroscopic methods and by comparison with those reported in the literature. Their anti-neuroinflammatory activities were evaluated by inhibitory effects on pro-inflammatory mediators [e.g. nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha)] in lipopolysaccharide (LPS)-activated BV-2 cells.Results: One new and sixteen known lignans were isolated and characterized. The absolute configuration of the new lignan, (7R,8S)-9-acetyl-dehydrodiconiferyl alcohol (1), was elucidated by a combination of 1D/2D NMR techniques and the Electronic Circular Dichroism (ECD) spectroscopy based on the empirical helicity rules. The anti-neuroinflammatory bioassay showed that compounds 1, (7R,8S)-dehydrodiconiferyl alcohol (2), erythro-guaiacylglycerol-beta-coniferyl ether (5), and threo-guaiacylglycerol-13-coniferyl ether (6) displayed significant inhibitory effects on NO production. Among them, neolignans 1 and 2 exhibited more potent activities than the positive control (N-G-monomethyl-r-arginine, L-NMMA), with an IC50 value of 9.3 and 3.9 mu M, respectively. Moreover, both I and 2 were also found to concentration-dependently suppress the TNF-a release in LPS-stimulated BV-2 cells.Conclusion: The results revealed that lignans are the major components of Chuan-Mu-Tong, and their anti-neuroinflammatory activities strongly support the traditional application of this herb medicine on inflammation. Moreover, the dihydrobenzo[b]furan neolignans 1 and 2 as well as Caulis clematidis armandii could be further exploited as new therapeutic agents to treat inflammation-mediated neurodegenerative and aging-associated diseases. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 鹤峰铁线莲 Clematis armandii