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Bioactive metabolites from Phoma species, an endophytic fungus from the Chinese medicinal plant Arisaema erubescens

作者 Wang, Li-Wei; Xu, Bai-Ge; Wang, Jia-Ying; Su, Zhen-Zhu; Lin, Fu-Cheng; Zhang, Chu-Long; Kubicek, Christian P.

期刊 APPLIED MICROBIOLOGY AND BIOTECHNOLOGY , FEB 2012,3:93

DOI 10.1007/s00253-011-3472-3 PubMed 21814808

摘要 Through bioassay-guided fractionation, the EtOAc extract of a culture broth of the endophytic fungus Phoma species ZJWCF006 in Arisaema erubescens afforded a new alpha-tetralone derivative, (3S)-3,6,7-trihydroxy-alpha-tetralone (1), together with cercosporamide (2), beta-sitosterol (3), and trichodermin (4). The structures of compounds were established on the basis of spectroscopic analyses. Compounds 1, 2, and 3 were obtained from Phoma species for the first time. Additionally, the compounds were subjected to bioactivity assays, including antimicrobial activity, against four plant pathogenic fungi (Fusarium oxysporium, Rhizoctonia solani, Colletotrichum gloeosporioides, and Magnaporthe oryzae) and two plant pathogenic bacteria (Xanthomonas campestris and Xanthomonas oryzae), as well as in vitro antitumor activities against HT-29, SMMC-772, MCF-7, HL-60, MGC80-3, and P388 cell lines. Compound 1 showed growth inhibition against F. oxysporium and R. solani with EC50 values of 413.22 and 48.5 mu g/mL, respectively. Additionally, compound 1 showed no cytotoxicity, whereas compound 2 exhibited cytotoxic activity against the six tumor cell lines tested, with IC50 values of 9.3 +/- 2.8, 27.87 +/- 1.78, 48.79 +/- 2.56, 37.57 +/- 1.65, 27.83 +/- 0.48, and 30.37 +/- 0.28 mu M, respectively. We conclude that endophytic Phoma are promising sources of natural bioactive and novel metabolites.

涉及物种 一把伞南星 Arisaema erubescens