---

Screening of a Natural Feed Additive Having Anti-viral Activity against Influenza A/H5N1

作者 Lee, Jang-Hyun; Kwon, Su Min; Seo, Sang Heui; Park, Young-Seo; Kim, Young-Bong; Kim, Soo-Ki; Paik, Hyun-Dong

期刊 KOREAN JOURNAL FOR FOOD SCIENCE OF ANIMAL RESOURCES , OCT 2008,4:28

DOI 10.5851/kosfa.2008.28.4.512

摘要 To search for anti-H5N1 influenza virus agent, the anti-viral activity of methanol and aqueous extracts from thirty medicinal plants were examined in this study. The plant material (30 g) was extracted with methanol (300 mL) for 24 hr at room temperature. Methanol extracts were filtered and evaporated, then freeze-dried. Aqueous extracts were prepared with dried plant material (30 g) and hot distilled water (300 mL). After 3 hr, the aqueous extracts were filtered and evaporated, then lyophilized. Extracts prepared from different plants were tested the antiviral activity against influenza viruses [A/Vietnam/1194/04 (H5N1)-NIBRG-14] using the hemagglutination (HA) assay. Among the test plants, Asarum sieboldii was found to be a potent inhibitor of H5N1 influenza virus in MDCK cell culture. Virus titers were 7 log, whereas with methanol extract of Asarum sieboldii for 48 hr titers were 3 log, indicating that methanol extract of Asarum sieboldii inhibited the H5N1 influenza viruses from the infected cells.

涉及物种 汉城细辛 Asarum sieboldii