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Chronic administration tetrahydroxystilbene glucoside promotes hippocampal memory and synaptic plasticity and activates ERKs, CaMKII and SIRT1/miR-134 in vivo

Authors Chen, Tao; Yang, Yuan-Jian; Li, Yan-Kun; Liu, Jing; Wu, Peng-Fei; Wang, Fang; Chen, Jian-Guo; Long, Li-Hong

Journal JOURNAL OF ETHNOPHARMACOLOGY , AUG 22 2016,:190

DOI 10.1016/j.jep.2016.06.012 PubMed 27275773

Abstract Ethnopharmacological Relevance: Polygonum multiflorum Thunb is a traditional Chinese medicine with anti-aging effect. 2,3,5,4''-tetrahydroxystilbene-2-0-beta-D-glucoside (TSG) is generally considered as the main active component in Polygonum multiflorum Thunb. However, the effect of TSG on memory in adult is unclear till now.Aim of study: 2,3,5,4''-tetrahydroxystilbene-2-0-beta-D-glucoside (TSG) is a polyphenols compound from Polygonum multiflorum Thunb. The present study aimed to evaluate the effect of chronic administration of TSG on hippocampal memory in normal mice.Materials and methods: Behavioral test, electrophysiology and golgi staining were used to evaluate the effect of TSG on hippocampus-dependent memory and synaptic plasticity. Western blotting was used to determine the expression of ERK1/2, CaMKII, and SIRT1. Real-time quantitative PCR was explored to measure miR-134.Results: It was found that TSG enhanced hippocampus-dependent contextual fear memory and novel object recognition, facilitated hippocampal LTP and increased dendrite spine density in the CA1 region of hippocampus. TSG obviously promoted the phosphorylations of ERK1/2, CaMKII, CREB and the expression of BDNF in the hippocampus, with upregulation of silent information regulator 1 (SIRT1) and downregulation of miR-134.Conclusions: Chronic administration of TSG promotes hippocampal memory in normal mice, suggesting that supplementary of TSG might serve as an enhancement of memory. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

Species covered 何首乌 Fallopia multiflora