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Large-scale separation of salvianolic acid B from the Chinese medicinal plant Salvia miltiorrhiza by pHl-zone-refining countercurrent chromatography

Authors Wang, Xiao; Geng, Yanling; Li, Fuwei; Liu, Jianhua

Journal JOURNAL OF SEPARATION SCIENCE , DEC 2007,18:30

DOI 10.1002/jssc.200700167 PubMed 17924368

Abstract pH-Zone-refining countercurrent chromatography was successfully applied to the separation of salvianolic acid B from the Chinese medicinal plant, Salvia miltiorrhiza Bunge, using a multilayer coil. planet centrifuge. A 2.0 g quantity of sample was separated using the following two-phase solvent system: methyl tert-butyl ether (MtBE)water, 10 mM TFA in organic stationary phase and 10 mM ammonia in aqueous mobile phase. The obtained fractions were analyzed by HPLC and ESI-MS. The separation yielded 572 mg of the main component of salvianolic acid B with a purity of 94.1%.

Species covered 丹参 Salvia miltiorrhiza