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Preparative Separation of Methylswertianin, Swerchirin and Decussatin from the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography

作者 Jia, Jing; Chen, Tao; Wang, Ping; Chen, Guichen; You, Jinmao; Liu, Yongjun; Li, Yulin

期刊 PHYTOCHEMICAL ANALYSIS , JUL-AUG 2012,4:23

DOI 10.1002/pca.1362 PubMed 22052665

摘要 Introduction Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available. Objective To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC). Methodology A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5?mL/min, a rotation speed of 800?rpm and a temperature of 25 degrees C. Results Using the described method, 8?mg of methylswertianin, 21?mg of swerchirin and 11?mg of decussatin with purities of over 98% could be isolated from a 150?mg crude sample. They were identified by 1H-NMR and 13?C-NMR analysis. Conclusion Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC. Copyright (C) 2011 John Wiley & Sons, Ltd.

涉及物种 川西獐牙菜 Swertia mussotii