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Anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea Javanica

Authors Yang, Jianhong; Li, Shucai; Xie, Caifeng; Ye, Haoyu; Tang, Huan; Chen, Lijuan; Peng, Aihua

Journal JOURNAL OF ETHNOPHARMACOLOGY , MAY 20 2013,2:147

DOI 10.1016/j.jep.2013.03.034 PubMed 23538165

Abstract Ethnopharmacological relevance: The seeds of Brucea javanica (L) Men. (Yadanzi in Chinese) have been used for the treatment of inflammation, dysentery, malaria, and cancer in Chinese traditional medicine. However, the anti-inflammatory mechanism of Brucea javanica has not been fully elucidated. This study examined the anti-inflammatory activity of ethyl acetate fraction of the seeds of Brucea javanica (EA-BJ) in vitro and in vivo.Materials and methods: The anti-inflammatory activity of EA-BJ and its ability to modulate the production of NO, PGE2, TNF-alpha, IL-1 beta, IL-6 and IL-10 inflammatory mediators in lipopolysaccharide-activated RAW 264.7 macrophage were evaluated. Moreover, the anti-inflammatory activity of EA-BJ was also in vivo assayed by carrageenan induced paw edema in mice.Results: In vitro assays showed remarkable anti-inflammatory activity of EA-BJ, through the inhibition of production of NO, PGE2, TNF-alpha, IL-1 beta and IL-6 inflammatory mediators and induction of production of IL-10 anti-inflammatory cytokine. In vivo assays showed anti-inflammatory activity for decrement of the paw edema in carrageenan induced paw edema test.Conclusion: The results obtained in vitro and in vivo showed that possible anti-inflammatory effects of EA-BJ may be attributed to inhibition pro-inflammatory mediators production, NO, PGE2, TNF-alpha, IL-1 beta and IL-6 and to increase production of IL-10 anti-inflammatory cytokine. The seeds of Brucea javanica may thus prove beneficial in the treatment of inflammatory diseases. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

Species covered 鸦胆子 Brucea javanica