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Identification of antihyperlipidemic constituents from the roots of Rubia yunnanensis Diels

作者 Gao, Yang; Su, Yaping; Huo, Yayu; Mi, Jie; Wang, Xinrui; Wang, Zhigang; Liu, Ying; Zhang, Hailong

期刊 JOURNAL OF ETHNOPHARMACOLOGY , SEP 11 2014,2:155

DOI 10.1016/j.jep.2014.07.027 PubMed 25064834

摘要 Ethnopharmacological relevance: The roots of Rubia yunnanensis Diels (Rubiaceae) have been used as an alternative for Rubia cordifolia for the treatment of various diseases including cardiovascular disease and metabolic disease for a long history in traditional Chinese medicine. To evaluate antihyperlipidemic activity of the roots of Rubia. yunnanensis Diels and to identify active compounds from the active fraction.Material and methods: Antihyperlipidemic effects of extract and compounds of the roots of Rubia yunnanensis were studied in HepG2 cells and in vivo model in olive oil-loaded mice. The isolation of compounds was conducted using various chromatographic methods and the structures of the isolated compounds were elucidated by NMR and MS techniques.Results: Methanol extract and ethyl acetate fraction of Rubia yunnanensis potently decreased the triglycerides accumulation in HepG2 cells and the methanol extract of Rubia yunnanensis (125, 250 and 500 mg/kg) significantly inhibited the TG elevation in a dose dependent manner in olive oil-loaded mice. Through various chromatographic methods, twenty-three compounds (1-23) were isolated in which arborinane-type triterpenoids and a free anthraquinone potently inhibited TG levels in HepG2 cells.Conclusion: Arborinane-type triterpenoids, especial rubiarbonone C (16) and an anthraquinone, 2-methyl-1, 3, 6-trihydroxy-9, 10-anthraquinone (MTHA) (22) were identified as the main active compounds responsible for antihyperlipidemic activity. Based on these findings, we proposed that the extract of Rubia yunnanensis and its active components, arborinane-type triterpenoids and the free anthraquinone might be beneficial in the treatment and prevention of hyperlipidemic disease. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 茜草 Rubia cordifolia 紫参 Rubia yunnanensis 茜草属 Rubia