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Bacterial Diversity in Roots, Stems, and Leaves of Chinese Medicinal Plant Paris polyphylla var. yunnanensis

Authors Liu, Tian-Hao; Zhou, Yin; Tao, Wen-Cong; Liu, Yang; Zhang, Xiao-Mei; Tian, Shou-Zheng

Journal POLISH JOURNAL OF MICROBIOLOGY , 2020,1:69

DOI 10.33073/pjm-2020-012 PubMed 32189484

Abstract The root of Paris polyphylla var. yunnanensis, a famous and endangered traditional Chinese herb, has a significant medicinal value. The aim of this study was to analyze the composition and functional characteristics of bacterial endophytes in roots, stems, and leaves of P. poly-phylla var. yunnanensis. The 16S rRNA gene sequencing and functional prediction of bacterial endophytes in roots, stems, and leaves of P. polyphylla var. yunnanensis were conducted. The Chao and Shannon indices of the bacteria in roots were significantly higher than those in stems and leaves. The dominant endophyte phyla were Cyanobacteria, Proteobacteria, Bacteroidetes, Firmicutes, and Actinobacteria. The main genera detected in roots were unclassified Cyanobacteria, Rhizobium, Flavobacterium, and Sphingobium; the main genera in stems were norank_c_Cyanobacteria, Bacillus, and Pseudomonas-, the main genera in leaves were norank_c_Cyanobacteria and Rhizobium. The microbiota in roots was particularly enriched in functional categories extracellular structures and cytoskeleton compared with stems and leaves (p <0.05). Our study reveals the structural and functional characteristics of the endophytic bacteria in roots, stems, and leaves of P. polyphylla var. yunnanensis, which aids in the scientific understanding of this plant.

Species covered 宽瓣重楼（变种） Paris polyphylla var. yunnanensis