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EXTRACTION AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHODS FOR THE GAMMA-LACTONES PARTHENOLIDE (CHRYSANTHEMUM PARTHENIUM BERNH), MARRUBIIN (MARRUBIUM-VULGARE L) AND ARTEMISININ (ARTEMISIA-ANNUA L)

作者 REY, JP; LEVESQUE, J; POUSSET, JL

期刊 JOURNAL OF CHROMATOGRAPHY , JUL 10 1992,1:605

DOI 10.1016/0021-9673(92)85036-S

摘要 A low-pressure liquid chromatographic method using silica gel 60 with hexane-chloroform-ethyl acetate proportions varying from 80:20:0 to 80:20:6 (v/v/v) as eluent is described as a simple and inexpensive process for the isolation of the gamma-lactones parthenolide, marrubiin and artemisinin from the aerial parts of feverfew, white horehound and quinghao, respectively. A selective high-performance liquid chromatographic (HPLC) method using a Superspher Si 60 column with hexane-dioxane (85:15, v/v) for elution of parthenolide and marrubiin and (90:10, v/v) for artemisinin and UV detection at 210 nm for parthenolide and artemisinin and 225 nm for marrubiin is described that allows the determination of these gamma-lactones in plants. Compared with the HPLC method, the extraction yield of the large-scale process was calculated to be 72.3, 78.4 and 74.1% of the total amount of parthenolide, marrubiin and artemisinin, respectively, contained in each plant.

涉及物种 蒿属 Artemisia