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DEVELOPMENT AND CHARACTERIZATION OF POLYMORPHIC MICROSATELLITE MARKERS IN DYSOSMA PLEIANTHA (BERBERIDACEAE)

Authors Guan, Bi-Cai; Gong, Xi; Zhou, Shi-Liang

Journal AMERICAN JOURNAL OF BOTANY , AUG 2011,8:98

DOI 10.3732/ajb.1100107 PubMed 21821582

Abstract Premise of the study: The development of compound microsatellite markers was conducted in Dysosma pleiantha to investigate genetic diversity and population genetic structure of this threatened medicinal plant.Methods and Results: Using the compound microsatellite marker technique, 14 microsatellite markers that were successfully amplified showed polymorphism when tested on 38 individuals from three populations in eastern China. Overall, the number of alleles per locus ranged from 2 to 14, with an average of 7.71 alleles per locus.Conclusions: These results indicate that these microsatellite markers are adequate for detecting and characterizing population genetic structure and genetic diversity in Dysosma pleiantha.

Species covered 六角莲 Dysosma pleiantha