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Isolation and characterization of 18 polymorphic microsatellite markers for the Female Ginseng Angelica sinensis (Apiaceae) and cross-species amplification

作者 Lu, Youyuan; Cheng, Tao; Zhu, Tiantian; Jiang, Dan; Zhou, Shiliang; Jin, Ling; Yuan, Qingjun; Huang, Luqi

期刊 BIOCHEMICAL SYSTEMATICS AND ECOLOGY , AUG 2015,:61

DOI 10.1016/j.bse.2015.07.013

摘要 Angelica sinensis (Apiaceae) is an endangered alpine herb that is widely used as a medicinal plant in traditional Chinese medicine (TCM). Wild populations of A. sinensis have become quite rare in China. Thus, population genetics studies of this species are urgently needed for its effective conservation and sustainable use. However, to date, no microsatellite loci have been isolated in A. sinensis. To address this issue, we isolated 18 polymorphic loci and genotyped 120 individuals collected from 6 populations. The number of alleles per locus ranged from 1.2 to 5.5, and the average was 2.4. The observed and expected heterozygosity per locus for a population varied, respectively, from 0.000 to 0.983 (averaged at 0.198) and from 0.066 to 0.661 (averaged at 0.333). Deviation from the Hardy-Weinberg equilibrium (p < 0.01) was observed for 4 to 14 loci in various populations. These microsatellite markers were cross-amplified in 10 species affinis, and 7 loci were successfully amplified in all species. These microsatellite markers are useful for genetic studies, the conservation management of A. sinensis, and identification of A. sinensis. (C) 2015 Elsevier Ltd. All rights reserved.

涉及物种 当归 Angelica sinensis