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Optimizing the compatibility of paired-herb in an ancient Chinese herbal decoction Kai-Xin-San in activating neurofilament expression in cultured PC12 cells

作者 Yan, Lu; Xu, Sherry L.; Zhu, Kevin Y.; Lama, Kelly Y. C.; Xin, Guizhong; Maiwulanjiang, Maitinuer; Li, Ning; Dong, Tina T. X.; Lin, Huangquan; Tsim, Karl W. K.

期刊 JOURNAL OF ETHNOPHARMACOLOGY , MAR 13 2015,:162

DOI 10.1016/j.jep.2014.12.049 PubMed 25560671

摘要 Ethnopharmacological relevance: Kai-Xin-San (KXS), a well-known traditional Chinese herbal decoction, has been widely used to treat mental depression and memory loss in China. It has a combination of four herbs: Ginseng Radix et Rhizoma (GR; root and rhizome of Panax ginseng C.A. Mey.), Polygalae Radix (PR; root of Polygala tenuifolia Wild.), Acori Tatarinowii Rhizoma (ATR; rhizome of Acorus tatarinowii Schott), and Poria (PO; sclerotium of Poriacocos (Schw.) Wolf), from which a pairing of two herbs was considered as paired-herb, such as the pairing of GR-PR and ATR-PO. The depression-induced neural cell loss is one of the major pathogenesis in depression. Here, an optimized ICXS by changing the ratio of paired-herbs in KXS was demonstrated aiming at promoting neural cell differentiation.Materials and methods: Quantitative assessment of chemical markers in each herbal extract was determined by LC-MS. Promoters of neurofilaments, NF68 and NF200, linked with luciferase reporter gene (pNF68-Luc and pNF200-Luc) were applied in cultured pheochromocytoma (PC12) cells to study the transcriptional activation of each herbal extract. The effect of GR-PR and ATR-PO in improving NF promoter activity was analyzed by Compusyn software. The activation of PICA was indicated.Results: In PC12 cells, an optimized KXS named KXS1:5 having 1:5 of GR-PR:ATR-PO had greater capability in promoting the expression of neurofilament. The synergistic effect of GR-PR and ATR-PO on the improved efficiency was further determined. Moreover, the treatment of H89, a PICA inhibitor, significantly inhibited the induced NF promoter activity.Conclusion: These results indicated an optimized 106 by optimizing the compatibility of paired-herb and this compatibility was proven to exert synergistic effect. Moreover, the underlying mechanism was mediated by a PICA signaling pathway. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 人参 Panax ginseng 远志 Polygala tenuifolia 远志属 Polygala 石菖蒲 Acorus tatarinowii