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The host immune enhancing agent Korean red ginseng oil successfully attenuates Brucella abortus infection in a murine model

作者 Reyes, Alisha Wehdnesday Bernardo; Huynh Tan Hop; Arayan, Lauren Togonon; Tran Xuan Ngoc Huy; Park, Soo Jong; Kim, Kwang Dong; Min, WonGi; Lee, Hu Jang; Rhee, Man Hee; Kwak, Yi-Seong; Kim, Suk

期刊 JOURNAL OF ETHNOPHARMACOLOGY , FEB 23 2017,:198

DOI 10.1016/j.jep.2016.12.026 PubMed 28012988

摘要 Ethnopharmacological relevance: Panax ginseng Meyer (Araliaceae), is one of the most valuable traditional Chinese medicines and is used for the treatment of various human diseases. In this study, we elucidated the protective mechanism of the essential oil from Korean red ginseng (RGO) against Brucella infection.Materials and methods: The effects of RGO on Brucella abortus viability, NO production, uptake and intracellular growth in macrophages were investigated. Mice were intraperitoneally infected with B. abortus and orally treated with RGO for 14 days. The weights and bacterial numbers from each spleen were monitored, and the sera were evaluated for cytokine production.Results: B. abortus viability was not affected, whereas NO production, internalization and intracellular replication were inhibited in RGO-treated macrophages. Bacterial adherence, F-actin polymerization and MAPK signaling protein phosphorylation (ERK1/2, JNK and p38 alpha) were reduced and the co-localization of B. abortus-containing phagosomes with LAMP-1 was augmented in RGO-treated cells compared to untreated cells. RGO displayed protective effects against cell damage by inhibiting nitrite production during B. abortus infection in macrophages. Moreover, the spleen weight and bacterial burden were lower in the RGO-treated group than in the control group. The uninfected RGO-treated mice displayed increased TNF-alpha and IFN-gamma production, whereas the B. abortus-infected RGO-treated mice showed reduced IL-10 production compared to the control.Conclusion: RGO exhibits protective effects against B. abortus infection in vitro and in vivo, which emphasize the beneficial effects of RGO in the prevention and treatment of brucellosis.

涉及物种 人参 Panax ginseng