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Polyphyllin D, a steroidal saponin from Paris polyphylla, inhibits endothelial cell functions in vitro and angiogenesis in zebrafish embryos in vivo

作者 Chan, Judy Yuet-Wa; Koon, Johnny Chi-Man; Liu, Xiaozhou; Detmar, Michael; Yu, Biao; Kong, Siu-Kai; Fung, Kwok-Pui

期刊 JOURNAL OF ETHNOPHARMACOLOGY , SEP 1 2011,1:137

DOI 10.1016/j.jep.2011.04.021 PubMed 21658438

摘要 Ethnopharmacological relevance: Angiogenesis, the process of blood vessel formation, is critical to tumour growth. The importance of angiogenesis in tumour development has lead to the development of antiangiogenic strategies to inhibit tumour growth. In this study, polyphyllin D (PD), an active component in Chinese herb, Paris polyphylla, was evaluated for its potential anti-angiogenic effects.Materials and methods: The inhibitory effects of PD on three important processes involved in angiogenesis, i.e. proliferation, migration and differentiation were examined using human microvascular endothelial cell line HMEC-1 by MTT assay, scratch assay and tube formation assay, respectively. Using zebrafish embryos as an animal model of angiogenesis, the anti-angiogenic effect of PD was further verified in vivo.Results: PD suppressed the growth of HMEC-1 cells at 0.1-0.4 mu M without toxic effects. At 0.3 mu M and 0.4 mu M, PD significantly inhibited endothelial cell migration and capillary tube formation. About 70% of the zebrafish embryos showed defects in intersegmental vessel formation upon treatment with PD at concentrations of 0.156 mu M and 0.313 mu M.Conclusion: The anti-angiogenic effects of PD have been explored in the study which implied a potential therapeutic development of PD in cancer treatment. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

涉及物种 七叶一枝花 Paris polyphylla