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Immunomodulatory activity of the seeds of Plantago asiatica L.

Authors Huang, Dan-Fei; Xie, Ming-Yong; Yin, Jun-Yi; Nie, Shao-Ping; Tang, Yong-Fu; Xie, Xiao-Mei; Zhou, Chao

Journal JOURNAL OF ETHNOPHARMACOLOGY , NOV 2009,7:27

DOI 10.1177/0734242X09103835 PubMed 19423590

Abstract Ethnopharmacological relevance: The seeds of Plantago asiatica L. were often used as a traditional Chinese medicine for some immunologically weak patients suffering from chronic illness. These uses could be related to immunomodulatory properties of the plant.Aim of the study: In this study, effects of extract of the seeds of Plantago asiatica L. (ES-PL) were investigated on the maturation of dendritic cells (DCs), which play significant role in primary immune system.Materials and methods: The phenotypes of DCs were analyzed by using flow cytometry while pliagocytosis was assessed by the uptake of FITC-dextran. Antigen presenting ability to allogeneically naive or syngeneically primed T lymphocytes was examined by the lymphocyte proliferation of mixed lymphocyte reaction (MLR). In addition, the level of chemokine receptor CCR7 mRNA was determined by RT-PCR.Results: DCs treated with ES-PL expressed higher levels of MHC class II molecules and major costimulatory molecules such as CD80 and CD86. Functional maturation of DCs treated with ES-PL was confirmed by decreased mannose receptor-mediated endocytosis and increased antigen presenting abilities to allogeneically naive or syngeneically primed T lymphocytes. The CCR7 mRNA expression in DCs treated with ES-PL was also enhanced.Conclusions: These results indicated that ES-PL could induce the maturation of murine DCs. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

Species covered 车前 Plantago asiatica