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MICROPROPAGATION OF CODONOPSIS PILOSULA (FRANCH.) NANNF BY AXILLARY SHOOT MULTIPLICATION

作者 Slupski, Wojciech; Tubek, Bogna; Matkowski, Adam

期刊 ACTA BIOLOGICA CRACOVIENSIA SERIES BOTANICA , APR 2018,:117

DOI 10.1016/j.micpath.2018.01.032 PubMed 29366865

摘要 Roots of Codonopsis pilosula (Franch.) Nannf. are among the most popular Chinese herbal medicines, exhibiting various beneficial activities which support immunity and stress resistance. The plant shows high intraspecific genetic variation. There is a need for effective vegetative propagation methods yielding high and sustainable quality. Here we report a micropropagation method using axillary shoot proliferation. Nodal segments from aseptically germinated plants were inoculated on modified MS media enriched with different concentrations of cytokinins: benzyladenine, kinetin (1, 4, 10 or 20 mu M) or thidiazuron (1, 4 or 8 mu M), with or without the auxin NAA (1 mu M). Axillary bud break was initiated most efficiently on media with 1 or 4 mu M BA and 1 mu M NAA. Shoot number increased markedly in subsequent cycles of harvesting and transfer to fresh 1 mu M BA and NAA medium, leading to the maximum 69 shoots (mean 38.16 +/- 4.35) from a single nodal explant in the fourth harvest. The shoots were successfully (>98% efficiency) rooted in MS medium containing high sucrose (60 g/L) and 5 mu M IAA, and acclimatized to soil cultivation with a survival rate of 90%. These results can be used to establish a simple and commercially viable protocol for mass propagation of C. pilosula for plantations or breeding.

涉及物种 党参 Codonopsis pilosula