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Taoren-dahuang herb pair reduces eicosanoid metabolite shifts by regulating ADORA2A degradation activity in ischaemia/reperfusion injury rats

Authors Li, Liu-Liu; Liu, Yan-Ru; Sun, Chen; Yan, Yong-Gang; Tang, Zhi-Shu; Sun, Jing; Li, Lu-Han; Song, Zhong-Xing; Wang, Dan-Yang; Li, Xiao-Hong; Chang, Ai-Bing; Yan, Ya-Feng; Gao, Jing; Peng, Liang

Journal JOURNAL OF ETHNOPHARMACOLOGY , OCT 5 2020,:260

DOI 10.1016/j.jep.2020.113014 PubMed 32473369

Abstract Ethnopharmacological relevance: Peach kernel (taoren: TR) is the dried mature seed of peach, Prunus persica (L.) Batsch, which belongs to the Rosaceae family. Rhubarb (dahuang: DH) is the dried root and rhizome of rhubarb (Rheum palmatum L., Rheum officinale Baill., or Rheum tanguticum Maxim. ex Balf.). TR-DH (TD) is a traditional Chinese medicine herb pair that promotes blood circulation and removes blood stasis. In recent years, TD has shown definite benefits in the cardio-cerebrovascular system, but its specific mechanism is not very clear.Aim of study: The purpose of this study was to explore the mechanism by which TD affects cerebral ischaemia/reperfusion (I/R) injury and to optimize the mixture ratio.Methods: The affected metabolic pathways in rat brain tissues after I/R were analysed by network pharmacology and verified with animal pharmacological experiments.Results: TD had a certain therapeutic effect on cerebral I/R injury. TD with a TR:DH ratio of 1:1 had the best therapeutic effect. Metabolic pathway analysis showed that the protective mechanism of TD against I/R injury involves mainly regulation of brain tissue ADORA2A protein levels and action on the arachidonic acid (AA) pathway.Conclusion: TD can ameliorate cerebral I/R injury by regulating ADORA2A degradation in the AA metabolic pathway to attenuate AA metabolic dysfunction and the inflammatory response.

Species covered 药用大黄 Rheum officinale 掌叶大黄 Rheum palmatum 鸡爪大黄 Rheum tanguticum