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Salvia miltiorrhiza (Danshen) inhibits L-type calcium current and attenuates calcium transient and contractility in rat ventricular myocytes

作者 Gao, Yonggang; Zhang, Kai; Zhu, Fengli; Wu, Zhonglin; Chu, Xi; Zhang, Xuan; Zhang, Ying; Zhang, Jianping; Chu, Li

期刊 JOURNAL OF ETHNOPHARMACOLOGY , DEC 2 2014,:158

DOI 10.1016/j.jep.2014.10.049 PubMed 25446591

摘要 Ethnopharmacological relevance: Salvia miltiorrhiza (SM, Danshen), a traditional Chinese herbal drug, has been widely used for hundreds of years to treat coronary artery disease.Materials and methods: We studied the effects of SM on the L-type Ca2+ current (ICa-L) with whole-cell patch-clamp technique in rat ventricular myocytes, and its influence on Ca2+ transient and contractility using video-based edge detection and dual excitation fluorescence photomultiplier systems as well.Results: Exposure to SM solution caused a concentration- and voltage-dependent blockade of ICa-L, and the dose of SM solution (10 g/l) decreased the maximal inhibitory effect of 35.2 +/- 1.2%. However, SM solution did not significantly change the current voltage relationship or reversal potential of ICa-L, nor did it altered the activation and inactivation gating properties of cardiac Ca2+ channels. Meanwhile, SM decreased the amplitude of myocyte shortening and the peak value of Ca2+ transient with a significant decrease in the time to 90% of the baseline (T-r), but the time to 10% of the peak (T-p) was not dramatically prolonged.Conclusions: The results indicated that SM significantly inhibited L-type Ca2+ channels, decreased [Ca2+](i); and contractility in adult rat cardiac myocytes. These findings may be relevant to the cardioprotective efficacy of SM. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 丹参 Salvia miltiorrhiza