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Sonchus oleraceus Linn protects against LPS-induced sepsis and inhibits inflammatory responses in RAW264.7 cells

Authors Chen, Lei; Lin, Xiujun; Xiao, Jianbo; Tian, Yuting; Zheng, Baodong; Teng, Hui

Journal JOURNAL OF ETHNOPHARMACOLOGY , MAY 23 2019,:236

DOI 10.1016/j.jep.2019.02.039 PubMed 30802614

Abstract Ethnopharmacological relevance: Sonchus oleraceus Linn (SOL) belongs to family of Asteraceae, is a traditional medicinal plant, which has been used to treat tumor, inflammatory diseases, infection and so on in Chinese folk culture.Aim of the study: This work investigated the influence of aqueous ethanol extract of whole plant of SOL and contribution of its main components on inflammationMethods and results: Oral administration of SOL (10 mg/kg) to mice reduced the expression of inflammatory cytokines including IL-6, IL-1 beta, and TNF-alpha, in the LPS-induced sepsis mouse model. Major phenolics in SOL were isolated and determined by HPLC. Results indicate that SOL at the concentration range from 25 to 100 g/mL and its main components, chlorogenic acid, caffeic acid (25-100 mu M) significantly reduced the pro-inflammatory cytokine IL-1 beta, IL-6, TNF-alpha, attenuated iNOS and COX-2 expression in LPS-stimulated Macrophages. In addition, western blot analysis showed SOL suppressed inducible nitric oxide synthase (iNOS) protein expression and the phosphorylation of extracellular signal-regulated kinase (ERK), p38, c-Jun N-terminal kinase (JNK).Conclusion: The underlying mechanism of anti-inflammation might be in according with the inhibition of MAPKs activation as well as down regulation of iNOS and cyclooxygenase-2 (COX-2).

Species covered 苦苣菜 Sonchus oleraceus