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Allobranchiibius huperziae gen. nov., sp nov., a member of Dermacoccaceae isolated from the root of a medicinal plant Huperzia serrata (Thunb.)

Authors Ai, Meng-Jie; Sun, Ye; Sun, Hong-Min; Liu, Hong-Yu; Yu, Li-Yan; Zhang, Yu-Qin

Journal INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY , OCT 2017,10:67

DOI 10.1099/ijsem.0.002284 PubMed 28920829

Abstract A Gram-stain-positive, non-spore-forming actinobacterial strain, designated CPCC 204077(T), was isolated from the surface sterilized root of a medicinal plant Huperzia serrata (Thunb.) collected from Sichuan Province, south-west China. The peptidoglycan type of strain CPCC 204077(T) was detected as A4a with an L-Lys-L-Ser-o-Asp interpeptide bridge. Galactose, glucose, rhamnose and ribose were the sugar compositions in the whole-cell hydrolysates. MK-8(H-4) was the only menaquinone. The polar lipid profile consisted of diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol, one unidentified phospholipid and one unidentified glycolipid. The major fatty acid was iso-C-16:0. The genomic DNA G+C content was 71.0 mol%. The phylogenetic tree based on 16S rRNA gene sequences showed that strain CPCC 204077(T) stood for a distinct lineage within the family Dermacoccaceae alongside the genera Branchiibius, Demetria and Barrientosiimonas, with the highest 16S rRNA gene sequence similarities to Branchiibius hedensis Mer 29717(T) (95.0 %), Calidifontibacter indicus PC IW02(T) (95.0 %), Barrientosiimonas humi 39(T) (94.9 %) and Demetria terragena HKI 0089(T) (94.7 %), and less than 94.7 % sequence similarities to all other species. Signature nucleotides in the 16S rRNA sequence showed that the strain contained the Dermacoccaceae family-specific 16S rRNA signature nucleotides and a genus-specific diagnostic nucleotide signature pattern. Combining the genotypic and phenotypic analyses, we propose that strain CPCC 204077(T) represents a novel species of a new genus in the family Dermacoccaceae with the name Allobranchiibius huperziae gen. nov., sp. nov. Strain CPCC 204077(T) (=NBRC 110719(T)=DSM 29531(T)) is the type strain of the type species.

Species covered 蛇足石杉 Huperzia serrata