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GC-MS method for determination and pharmacokinetic study of four phenylpropanoids in rat plasma after oral administration of the essential oil of Acorus tatarinowii Schott rhizomes

作者 Wang, Zhibin; Wang, Qiuhong; Yang, Bingyou; Li, Jing; Yang, Chunjuan; Meng, Yonghai; Kuang, Haixue

期刊 JOURNAL OF ETHNOPHARMACOLOGY , SEP 11 2014,2:155

DOI 10.1016/j.jep.2014.06.035 PubMed 25046827

摘要 Ethnopharmacological relevance: Acorus tatarinowii Schott (AT), belong to the family Araceae, is perennial herbaceous plant mainly present in China, Japan and India. The rhizomes of AT have been used as a famous traditional Chinese medicine for the treatment of central nervous system related diseases. Aim of the study: A selective, accurate and sensitive method using gas chromatography-mass spectroscopy (GC-MS) for the simultaneous determination and pharmacokinetic study of beta-asarone, alpha-asarone, elemicin and cis-methyl isoeugenol in rat plasma was developed and validated.Materials and methods: The GC-MS system was operated under selected ion monitoring (SIM) mode. The samples were prepared by protein precipitation with acetonitrile after being spiked with an internal standard (1-naphthol). The GC separation was achieved on a DB-1701 column (60 m x 0.25 mm ID, and 0.25 mu m film thickness).Results: The current GC/MS assay was validated for linearity, intra-day and inter-day precisions, accuracy, extraction recovery and stability. The analyte calibration curves were linear over a wide concentration range and the lowest limit of quantifications (LLOQ) were 5.53 ng/mL (beta-asarone), 6.50 ng/mL (alpha-asarone), 3.10 ng/mL (elemicin) and 7.60 ng/mL (cis-methyl isoeugenol). After oral administration 0.9 g /Kg of AT rhizomes, the maximum plasma concentration (Cmax) was 2508.6 +/- 498.7 ng/mL for beta-asarone, 257.5 +/- 37.1 ng/mL for alpha-asarone, 345.5 +/- 33.4 ng/mL for elerriicin and 452.7 +/- 59.1 ng/mL for cis-methyl isoeugenol, respectively. The time to reach the maximum plasma concentration (T-max) was 1.42 +/- 0.18 h for beta-asarone, 1.58 +/- 0.19 h for alpha-asarone, 1.67 +/- 0.24 h for elemicin and 1.75 +/- 0.38 h for cis-methyl isoeugenol, respectively.Conclusion: This paper described a simple, sensitive and validated GC-MS method for simultaneous determination of four phenylpropanoids in rat plasma after oral administration of the essential oil of AT rhizomes and investigated on their pharmacokinetics studies as well. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 石菖蒲 Acorus tatarinowii