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Use of DNA barcode sensu lato to identify traditional Tibetan medicinal plant Gentianopsis paludosa (Gentianaceae)

Authors Xue, Chun-Ying; Li, De-Zhu

Journal JOURNAL OF SYSTEMATICS AND EVOLUTION , MAY 2011,3:49

DOI 10.1111/j.1759-6831.2011.00127.x

Abstract Gentianopsis paludosa (Hook. f.) Ma (Gentianaceae) is an important species in Tibetan folk medicine commonly used to clear away the heat evils and toxic materials. A survey of market samples revealed that nine adulterant species, Gentianopsis barbata, G. contorta, G. grandis, Halenia elliptica, Lomatogonium macranthum, L. rotatum, Swertia angustifolia, S. bifolia and S. erythrosticta, are often marketed as G. paludosa. Methods to distinguish G. paludosa from its adulterants are limited by current morphological and chemical methods. DNA sequence analysis of the nuclear ribosomal DNA internal transcribed spacer region (ITS) was used in the differentiation of G. paludosa from the adulterant species. The data showed that the internal transcribed spacer regions differ significantly between G. paludosa and all nine adulterant species, so that they could be easily distinguished at the DNA level.

Species covered 椭圆叶花锚(原变种) Halenia elliptica 扁蕾 Gentianopsis barbata 湿生扁蕾(原变种) Gentianopsis paludosa