Optimization of DNA Micro-extraction Method of Saussurea medusa Maxim, A Rare Tibetan Medicinal Plant of Qinghai-Tibet Plateau

Authors Wang Jun; Hu Yanping; Wang Jianke; Shi Lin; Yang Wentao; Li Yi; Wang Li

Journal Molecular Plant Breeding , 2016,9:14

Abstract In this study, Genomic DNA of dried Saussurea medusa was extracted through CTAB-improved method, SDS-improved method and high salt low pH basic method, then optimized and screened a suitable method for DNA extraction of S. medusa. Results showed that the yield of genomic DNA extracted by CTAB-improved method (2% CTAB concentration, 1% PVP concentration, 2% beta-mercaptoethanol concentration) was higher than others. And two times of extraction with chloroform/isoamyl alcohol had got the better quality of DNA that met the requirements of ISSR analysis. On the basis of this, preprocessed the experimental materials and got a complete set of DNA extraction process of S. medusa dried herbs. This improved method can effectively remove the effect of secondary metabolites and consume less plant materials and it also can be applied to the germplasm identification and genetic diversity analysis of S.medusa.

Species covered 水母雪兔子 Saussurea medusa