Development of ten microsatellite loci for Gentiana crassicaulis (Gentianaceae)

作者 Li, Yong; Li, Lin-Feng; Chen, Guo-Qing; Ge, Xue-Jun

期刊 CONSERVATION GENETICS , 2010,1:30

摘要 Genetiana crassicaulis is one of famous Chinese medicinal plant. The over-collection for its root has caused its dramatic reduction. In order to devise adequate conservation and management strategies for this species, it is important to characterize its genetic diversity and understand its population structure. Here, 10 polymorphic microsatellite markers have been developed. AC/TG microsatellite was enriched by combining biotin capture method. Polymorphism of each locus was assessed in 30 individuals from six populations. The number of alleles ranged from 2 to 9 and the expected heterozygosity ranged from 0.32 to 0.78.

涉及物种 粗茎秦艽 Gentiana crassicaulis