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The fruit of Crataegus pinnatifida ameliorates memory deficits in beta-amyloid protein-induced Alzheimer''s disease mouse model

Authors Lee, Jihye; Cho, Eunbi; Kwon, Huiyoung; Jeon, Jieun; Jung, Chul Jong; Moon, Minho; Jeon, Mira; Lee, Young Choon; Kim, Dong Hyun; Jung, Ji Wook

Journal JOURNAL OF ETHNOPHARMACOLOGY , NOV 2019,6:17

DOI 10.1016/j.joim.2019.08.001 PubMed 31447352

Abstract Ethnopharmacological relevance: The fruit of Crataegus pinnatifida is a traditional medicine widely used as digestive drug in East Asia. Although Chinese herbal medicine used it for mental health, scientific evidence does not exist, yet.Aims of study: The aim of this study is to show that the ethanol extract of the fruit of Crataegus pinnatifida (CPE) has neuroprotective effect on Alzheimer'' disease model mice.Materials and methods: Intracerebroventricular injection of A beta was used to induce Alzheimer''s disease-like pathology. Passive avoidance and Y-maze tasks were used to examine the effect of CPE on memory impairrilents by A beta. Immunohistochemistry was used to examine the effect of CPE on glial activation. ThT assay was used to observe the effect of CPE on A beta aggregation. MTT and LDH release assays were utilized to examine effects of CPE on A beta-induced cytotoxicity.Results: CPE prevented memory deficit in A beta-induced memory impairment model. Moreover, CPE prevented glial activation in the hippocampus of A beta-injected model. In in vitro test, CPE inhibited A beta fibril formation in a concentration-dependent manner. CPE also caused disaggregation of A beta fibrils. Along with this, CPE blocked neuronal cell death induced by A beta.Conclusions: Collectively, these experimental findings demonstrated that CPE could be a candidate for development of AD therapy.

Species covered 山楂 Crataegus pinnatifida