Content Determination of Diosgenin in Paridis Rhizoma Medicinal Plants in Different Habitats of Three Gorges Reservoir

Authors Zhang Jing; Ding Bo; Qi Junsheng; Chen Xiuhong; Zhou Nong

Journal Chinese Journal of Information on Traditional Chinese Medicine , 2016,6:23

Abstract Objective To establish an HPLC method to determine the content of diosgenin in Paridis Rhizoma; To examine contents of diosgenin in different varieties of Paridis Rhizoma medicinal plants and from different habitats. Methods Shimadzu Inertsil ODS-C_(18) guard column (150 mm*4.6 mm, 5mum) was used in HPLC system; elution conditions were the mobile phase using 92% acetonitrile and 8% water (isocraticelution) at a flow rate of 1.0 mL/min with 203 nm detection wavelength. The temperature of the column oven was maintained at 30 ℃. Results Different varieties of Paridis Rhizoma medicinal plants and Paridis Rhizoma medicinal plants from different habitats contained diosgenin. The Paris polyphylla var. pseudothibetica in Yubei district had the highest content (6.813 7 mg/g); Paris polyphylla var. stenophylla in Chengkou County had the middle content (5.758 4 mg/g); Paris yunnanensis Franch in Shizhu county showed the lowest content (1.952 2 mg/g). The results showed that the obvious difference of chemical quality between various cultivated varieties of Paridis Rhizoma medicinal plants, which might be related with origin, variety and the cultivation technology, and so on. Conclusion The diosgenin contents of Paris polyphylla var. pseudothibetica and Paris polyphylla var. stenophylla were higher than that of varieties recorded in Chinese Pharmacopeia. Paris polyphylla var. pseudothibetica and Paris polyphylla var. stenophylla had important medical value.

Species covered 狭叶重楼(变种) Paris polyphylla var. stenophylla 宽瓣重楼（变种） Paris polyphylla var. yunnanensis