The genetic transformation of Pueraria as medicinal plant by Agrobacterium rhizogenes

作者 Liu, CF; Yu, SH; Li, L; Shi, HP; Pan, RC

期刊 ACTA BOTANICA SINICA , SEP 2000,9:42

摘要 Pueraria radix (the dried root of Pueraria plant) is known as a traditional Chinese drug. Hairy roots of Pueraria lobata (Willd.) Ohwi, P. lobata var. montana and P. phaseoloides (Roxb.) Benth. transformed by Agrobacterium rhizogenes (Riker et al.) Conn R1601 were developed directly from the surface of sterile leaves in vitro. The transformation frequency was 16.6%, 16.2% and 26.6%, respectively. All hairy roots in the three species displayed the typical phenotypes of rapid growth, highly branched and plagiotropism, and also exhibited hormone autotrophy and resistance to kanamycin. The genetic transformations were confirmed by opine paper electrophoretic analysis, rol gene PCR amplification and molecular hybridization.

涉及物种 葛 Pueraria lobata