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Phenolic metabolite profiles and antioxidants assay of three Iridaceae medicinal plants for traditional Chinese medicine She-gan by on-line HPLC-DAD coupled with chemiluminescence (CL) and ESI-Q-TOF-MS/MS

Authors Xie, Guo-Yong; Zhu, Yan; Shu, Pan; Qin, Xiao-Ying; Wu, Gang; Wang, Qi; Qin, Min-Jian

Journal JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS , SEP 2014,:98

DOI 10.1016/j.jpba.2014.05.008 PubMed 25106824

Abstract An on-line analysis method by HPLC-DAD coupled with chemiluminescence (CL) and ESI-Q-TOF-MS/MS was established for simultaneous detection and identification of antioxidants in three original plants of traditional Chinese medicine She-gan. Two new isoflavonoid glycosides, along with 48 known compounds, including isoflavonoid glycosides and their aglycones, xanthones, flavones and other phenolic compounds, were identified or tentatively identified from the rhizomes of three Iridaceae plants, namely, Belamcanda chinensis, Iris tectorum and Iris dichotoma, which were used as She-gan in China. Among those compounds, isoflavone glycosides of iristectorigenin A and its isomers exhibited obviously inhibit CL, which suggested their strong free radical scavenging activity. The chemometric methods dealing with the data gained by chromatographic and antioxidant activity profiles exhibited the similarities and differences of chemical constituents and antioxidant activities for three studied Iridaceae species. The results indicated that the established method might provide for qualitative and quantitative evaluation of the herbal medicines. (C) 2014 Elsevier B.V. All rights reserved.

Species covered 野鸢尾 Iris dichotoma 鸢尾 Iris tectorum 射干 Belamcanda chinensis