---

Effects of Salvia miltiorrhiza on neural differentiation of induced pluripotent stem cells

作者 Shu, Tao; Pang, Mao; Rong, Limin; Zhou, Wei; Wang, Juan; Liu, Chang; Wang, Xuan

期刊 JOURNAL OF ETHNOPHARMACOLOGY , APR 11 2014,1:153

DOI 10.1016/j.jep.2014.02.028 PubMed 24568774

摘要 Ethnopharmacological relevance: Salvia miltiorrhiza, a well-known traditional Chinese medicine, is commonly used to treat some neurological diseases because of its anti-oxidative, anti-inflammatory and anti-apoptotic properties. We investigate whether Salvia miltiorrhiza can improve the differentiation of induced pluripotent stem cells (iPSCs) into neurons in vitro, and promote iP5Cs-derived neural stem cells survival, integrate, and differentiation after their transplantation to the ischemic brain tissues.Materials and methods: Induced pluripotent stem cells were used to differentiate into neural stem cells, and further into neurons in induction medium with various concentrations of Salvia miltiorrhiza. The effects were assessed by immunofluorescence staining, quantitative reverse transcription-polymerase chain reaction (qRT-PCR) and Western blotting. iPSC-derived neural stem cells were transplanted into the brains of rats with middle cerebral artery occlusion, immunofluorescence staining was used to evaluate survival, integrate, and differentiation of grafted cells, the functional recovery of the animals was tested by the Longa scores and spontaneous motor activity.Results: Salvia miltiorrhiza (5 mu g/ml) significantly increased the gene and protein expression of Nestin compared with that in other groups. Microtubule-associated protein 2 (MAP2) expression in induction medium with 5 mu g/ml Salvia miltiorrhiza was significantly higher than that in the control group. After cells transplantation into the ischemic brain, more grafted MAP2 cells were found in Salvia miltiorrhiza-treated rats than others at 7 days. Salvia miltiorrhiza-treated rats showed the most remarkable functional recovery at 7 and 14 days.Conclusion: Salvia miltiorrhiza induces differentiation of induced pluripotent stem cells to differentiate into neurons efficiently. The plant provides neuroprotection to implanted cells and improves functional recovery after their transplantation to the ischemic brain tissues. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 丹参 Salvia miltiorrhiza