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Pharmacokinetic comparisons of five ephedrine alkaloids following oral administration of four different Mahuang-Guizhi herb-pair aqueous extracts ratios in rats

作者 Wei, Ping; Huo, Hui-ling; Ma, Qing-hai; Li, Han-cheng; Xing, Xue-feng; Tan, Xiao-mei; Luo, Jia-bo

期刊 JOURNAL OF ETHNOPHARMACOLOGY , AUG 8 2014,1:155

DOI 10.1016/j.jep.2014.05.065 PubMed 24929107

摘要 Ethnophannacological relevance: Herba Ephedra (Mahuang in Chinese), is derived from dried Ephedra sinica Stapf stems and has been widely used to treat the common cold, coughs, asthma, and edema for thousands of years. The Mahuang-Guizhi herb-pair is a famous formula composed of Mahuang and Ramulus Cinnamomi (Guizhi in Chinese, the dried twig of Cinnamomum cassia Presl.), used to improve pharmacological effects and reduce toxicity. In order to investigate the influence of Mahuang-Guizhi herb-pair ratios on bioavailability, the plasma pharmacokinetics profiles of five ephedrine alkaloids were compared following oral administration of four different ratios to rats.Materials and methods: Sprague-Dawley rats were randomly assigned to four groups and orally administered Mahuang-Guizhi (ratios 3:0; 3:1; 3:2; 3:4, w/w). Assays for five ephedrine alkaloids (ephedrine, pseudoephedrine, methylephedrine, norephedrine, and norpseudoephedrine) were developed and validated using ultra-high-performance liquid chromatography tandem mass spectrometry coupled with liquid-liquid extraction.Results: Key pharmacokinetic parameters of the five ephedrine alkaloids (maximal plasma concentration, mean residence time, and half-life) were significantly different (p < 0.05) after oral administration of Mahuang-Guizhi herb-pair ratios, as compared to those of Mahuang.Conclusion: Ephedrine alkaloid pharmacokinetic differences in rat plasma could help explain previous findings of pharmacological and toxicity differences between Mahuang and Mahuang-Guizhi herb-pair preparations. These results could facilitate future studies to increase the efficacy and decrease the toxicity of Mahuang and Guizhi. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

涉及物种 草麻黄 Ephedra sinica 肉桂 Cinnamomum cassia