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Identification of Crude Drugs from Chinese Medicinal Plants of the Genus Bupleurum Using Ribosomal DNA ITS Sequences

Authors Xie, Hui; Huo, Ke-ke; Chao, Zhi; Pan, Sheng-li

Journal PLANTA MEDICA , JAN 2009,1:75

DOI 10.1055/s-0028-1088334 PubMed 19016401

Abstract To determine the variation of rDNA ITS sequences in medicinal plants of the genus Bupleurum and identify corresponding DNA molecular markers associated with medicinally important members, ITS regions were amplified, sequenced, and analyzed by DNAssist version 2.2. We found that the homologous alignment of ITS sequences between members of the genus Bupleurum and associated out-groups was lower than 75%, while within-group alignment was greater than 87%. The conclusion can be drawn that ITS sequences can be used as reliable molecular markers for the identification of Radix Bupleuri.

Species covered 柴胡属 Bupleurum