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Ginseng-Aconite Decoction elicits a positive inotropic effect via the reverse mode Na+/Ca2+ exchanger in beating rabbit atria

作者 Cui, Hao Zhen; Kim, Hye Yoom; Kang, Dae Gill; Lee, Ho Sub

期刊 JOURNAL OF ETHNOPHARMACOLOGY , JUL 9 2013,2:148

DOI 10.1016/j.jep.2013.05.014 PubMed 23702039

摘要 Ethnopharmacological relevance: Ginseng-Aconite Decoction (GAD), a traditional oriental medicine composed of Panax ginseng C.A. Mey. (Araliaceae) and Aconitum carmichaeli Debx. (Ranunculaceae) has been used as treatment for cardiovascular diseases from Song Dynasty of China. The purpose of the present study was to elucidate the possible mechanisms of GAD-induced positive inotropic effect.Material and methods: GAD-induced changes in atrial dynamics and cAMP efflux were determined in isolated perfused beating rabbit atria.Results: GAD significantly increased atrial dynamics such as stroke volume, pulse pressure and augmented cAMP efflux in beating rabbit atria. The inotropic effect was significantly attenuated by pre-treatment with KB-R7943, a reverse mode Na+/Ca2+ exchanger blocker. The GAD-induced increase in atrial dynamics was also markedly inhibited by staurosporine, a non-selective protein kinase inhibitor, and partly blocked by KT5720, a selective PICA inhibitor. The effect of GAD on atrial dynamics was not altered by pre-treatment with propranolol, a beta-adrenergic receptor inhibitor, or diltiazem, an L-type Ca(2+)channel blocker. The phosphodiesterase (PDE) inhibitor 3-isobutyl-1-methylxanthine (IBMX) failed to modulate the GAD-induced increase in atrial dynamics, but markedly attenuated cAMP efflux in the beating atria.Conclusion: These results suggest that the GAD-induced positive inotropic effect in beating rabbit atria may be attributable to stimulation of the reverse mode Na+/Ca2+ exchanger, while PKA activity would, at least in part, be participated in the course. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

涉及物种 人参 Panax ginseng 乌头 Aconitum carmichaeli