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Evaluation of surfactant-assisted pressurized hot water extraction for marker compounds in Radix Codonopsis pilosula using liquid chromatography and liquid chromatography/electrospray ionization mass spectrometry

Authors Ong, ES; Len, SM

Journal JOURNAL OF SEPARATION SCIENCE , NOV 2003,17:26

DOI 10.1002/jssc.200301578

Abstract In the move towards the elimination of organic solvents in the extraction process in botanicals, a new method combining surfactant and pressurized hot water extraction (PWHE) with an applied temperature below the boiling point and lower pressure from 10 to 20 bar was developed for the analysis of marker compounds that are reasonably hydrophobic such as tetradeca-4E,12E-diene-8,10-diyne-1,6,7-triol and tetradeca-4E,12E-diene-8,10-diyne-1,6,7-triol-O-beta-D-glucoside in Radix Codonopsis pilosula (DangShen). Because reference substances for the proposed botanicals were not available, a method was developed to isolate the marker compounds in Radix Codonopsis pilosula. Other than surfactant-assisted PHWE, the marker compounds present in Radix Codonopsis pilosula were extracted using pressurized liquid extraction (PLE) with methanol and PHWE with a mixture of water/ethanol (80:20). The extracts were analyzed using liquid chromatography and liquid chromatography/electrospray ionization mass spectrometry. With surfactant-assisted PHWE, the effects of different added surfactants such as sodium dodecyl sulfate and Triton X-100 was studied. Surfactant assisted PHWE with Triton X-100 proved to be at least equivalent or better compared to Soxhlet extraction in terms of quantitative analysis of marker compounds in Radix Codonopsis pilosula. The method precision was less than 8% (RSD, n = 6). The presence of surfactants in PHWE was found to enhance the solubility of target compounds naturally occurring in medicinal plants.

Species covered 党参 Codonopsis pilosula