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In vitro Germination,Callus Induction and Primary Haustorium Organogenesis in the Parasitic Plant Cynomorium songaricum

Authors 岳鑫; 陈贵林

Journal Bulletin of Botanical Research , MAY 30 2007,1:112

DOI 10.1016/j.jep.2007.02.026 PubMed 17418512

Abstract Cynomorium songaricum Rupr. is an obligate parasitic plant widely used as traditional Chinese medicine and Mongolian medicine. Here, we firstly describe protocols for in vitro germination, callus induction and haustorium organogenesis in C. songaricum Rupr. In this study, adequate concentrations of gibberellic acid (GA_3), in combination with other plant growth regulators in the medium, promoted embryo development and germination of C. songaricum seeds. The highest callus induction rate from seeds (13.7%) after a 40 d incubation was obtained with Bs medium adding 2,4-dichlorophenoxyacetic acid(2,4-D; 1.0 mg·L~(-1)), kinetin (KT;0.5 mg·L~(-1)) and GA_3(1.0 mg·L~(-1). This resulted in callus formation in 13.7%3.1% of seeds. Addition of 2, 4-D(0.5 mg·L~(-1)) and KT(0.25 mg·L~(-1)) yielded highest haustorium organogenesis from calluses. Some primary haustorium branched to form adventitious roots of 3-4 cm in length. Subsequently, the tip of each adventitious root formed a nascent primary haustorium, which was then branched out into adventitious roots. The role of auxins(2, 4-D in this study) in the formation of primary haustorium and adventitious roots from seed callus in C. songaricum was also discussed.

Species covered 锁阳 Cynomorium songaricum