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Dual effects of lipophilic extract of Salvia miltiorrhiza (Danshen) on catecholamine secretion in cultured bovine adrenal medullary cells

作者 Mao, Haoping; Zhang, Han; Wang, Hong; Wang, Yuefei; Zhao, Fenrong; Hu, Limin; Yanagihara, Nobuyuki; Gao, Xiumei

期刊 JOURNAL OF ETHNOPHARMACOLOGY , AUG 17 2009,1:125

DOI 10.1016/j.jep.2009.06.010 PubMed 19549564

摘要 Ethnopharmacological relevance: Salvia miltiorrhiza (Danshen) is a well known traditional Chinese herb, which has been used widely in China for the treatment of cardiovascular diseases in clinic.Aim of this study: The aim of the present study is to clarify the effects of lipophilic extract: of Salvia miltiorrhiza (LESM) on catecholamine (CA) secretion, a traditional Chinese medicine used widely for the treatment of cardiovascular diseases in China.Materials and methods: LESM was evaluated for its effects on CA secretion using HPLC-ECD method. The effects of LESM on Na-22(+) influx and intracellular calcium ([Ca2+](i)) were also investigated.Results: Our results showed that LEMS directly stimulated basal CA secretion in an extracellular Ca2+-dependent manner. And the stimulation was not affected by combination of hexamethonium (Hex), an inhibitor of nAChR. LESM also directly elevated [Ca2+]. In addition, using selective blockers; of voltage-dependent Ca2+ channels, such as nitrendipine (for L-type), omega-agatoxin-IVA (for P-type) and omega-conotoxin-GVIA (for N-type), it was found that nitrendipine suppressed the elevation of [Ca2+](i) induced by LESM, but not omega-agatoxin-IVA or omega-conotoxin-GVIA. Compared with acetylcholine (ACh) only, however, combination of LESM with ACh inhibited the raise of CA secretion, Na-22(+) influx and [Ca2+](i) in a concentration-dependent manner. Furthermore, LESM also inhibited CA secretion induced by veratridine (Ver), and 56 mM K+ at concentrations similar to those for [Ca2+](i) rise. one of the lipophilic active compounds, cryptotanshione (Cryp), also had the same effects on CA secretion with LESM.Conclusions: All these findings suggest that LESM exerts dual effects on CA secretion in cultured bovine adrenal medullary cells. LESM exerts antagonistic effects on nAChR, voltage-dependent Na+ and Ca2+ channels, whereas it is an agonist of L-type Ca2+ channel when it used alone. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

涉及物种 丹参 Salvia miltiorrhiza