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Chemical Composition of Three Polysaccharides From Gynostemma pentaphyllum and Their Antioxidant Activity in Skeletal Muscle of Exercised Mice

作者 Chi, Aiping; Tang, Liang; Zhang, Jing; Zhang, Kunru

期刊 INTERNATIONAL JOURNAL OF SPORT NUTRITION AND EXERCISE METABOLISM , DEC 2012,6:22

DOI 10.1123/ijsnem.22.6.479 PubMed 22899810

摘要 Purpose: To investigate the effects of polysaccharide from Gynostemma pentaphyllum on antioxidant activity in skeletal muscle of mice exercised to exhaustion. Methods: Three polysaccharide fractions were obtained from G. pentaphyllum polysaccharide (GPP) and termed GPP I-a. GPP2-b, and GPP3-a. Gas chromatography (GC) and infrared spectrum of the polysaccharides were determined. The fractions were orally administrated to mice once daily for 1 wk. The exercise time to exhaustion was assessed using a forced swim test of mice after a week. The glucose, creatine phosphokinase, and lactic dehydrogenase in serum; the activity of superoxide dismutase and glutathione peroxidase; and the levels of malondialdehyde (MDA) and glycogen in muscle were determined. Results: The results of GC demonstrated that GPP1-a, GPP2-b, and GPP3-a were composed of different monosaccharides with distinct molar ratios. Infrared spectrum showed that the main typicals of GPP1-a and GPP2-b were beta-configuration and the main typical of GPP3-a was alpha-configuration. Among the 3 fractions of GPP, GPP1-a administration significantly prolonged exercise time to exhaustion of mice, increased glycogen level and some of antioxidant enzyme activities, and decreased MDA level in muscle. Conclusions: The mechanism by which GPP1-a prolonged exercise time to exhaustion in mice may be associated with scavenging reactive oxygen species excessively produced and further increasing glycogen levels in skeletal muscle.

涉及物种 绞股蓝 Gynostemma pentaphyllum