Genetic diversity among five species medicinal plants of Lonicera L.in Guizhou Province by SRAP molecular markers analysis

Authors Pan Luchang; Zhao Zhi; He Yun; Li Yuanyuan; Liu Hongchang; Feng Tingting; Zhou Ying

Journal Journal of Southern Argiculture , 2018,12:49

Abstract 【Objective】Analysis of genetic diversity of five species medicinal plants of Lonicera L.in Guizhou Province was conducted.It provided reference for the identification,fine breed selection and utilization of medicinal plants of Lonicera L.【Method】Collecting the tender shoots of six medicinal plants samples of Lonicera L.as test materials in Guizhou Province,their genetic diversity was studied by SRAP marker,and the genetic similarity coefficient among the materials was calculated by NTSYS-pc2.1.The UPGMA method was used for cluster analysis.【Result】From the 154 pairs of SRAP primer combinations,15 pairs of primer combinations with good reproducibility,clear bands and rich polymorphism,and amplified bands(100-1000 bp)≥5 were screened.A total of 197 bands were amplified by 15 pairs of SRAP primers,among them there were 178 polymorphic bands.The percentage of polymorphic bands was 90.36%.On average,each pair of primers amplified 11.87 polymorphic bands.Among them,the primer combination of Me8-Em10 amplified the most polymorphic bands(16),but the primer combination of Me2-Em2 amplified the least polymorphic bands(8).The number of amplified bands of primer-combined Me4-Em2 was 15 and all were polymorphic bands,the number of amplified bands of five medicinal plants samplesof Lonicera L.was different.Cluster analysis results displayed,the similarity coefficient between the sixmedicinal plants samples of Lonicera L.was 0.4213-0.8477,and the average similarity coefficient was 0.5672.Among them,the genetic similarity coefficient between wild L.japonica Thunb.and cultivated L.japonica Thunb.was the highest(0.8477),indicating that the genetic relationship was the closest,and the genetic similarity coefficient of L.macranthoides Hand.-Mazz.and wild L.japonica Thunb.was the smallest(0.4213),indicating that the genetic relationship was the farthest.At the genetic similarity coefficient of 0.4873,six medicinal plants samples of Lonicera L.were divided into two categories.The first category included L.macranthoides Hand.-Mazz.,L.hypoglauca Miq.and L.fulvotomentosa Hsu et S.C.Cheng,all of which were the base plants of the Flos Lonicerae.The second category included wild L.japonica Thunb.cultivated L.japonica Thunb.and L.japonica.var.chinensis(Wats.)Bak.,which were the base plants of Lonicerae japonica.【Conclusion】The five species medicinal plants of Lonicera L.have rich genetic diversity in Guizhou Province.SRAP molecular markers can effectively analyze their genetic diversity and genetic relationship.The SRAP primer combination of Me4-Em2 can be used to identify five species medicinal plants of Lonicera L.

Species covered 忍冬属 Lonicera