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Tanshinones isolated from the rhizome of Salvia miltiorrhiza inhibit passive cutaneous anaphylaxis reaction in mice

Authors Trinh, Hien Trung; Chae, Sun Ju; Joh, Eun-Ha; Son, Kun Ho; Jeon, Su Jin; Kim, Dong-Hyun

Journal JOURNAL OF ETHNOPHARMACOLOGY , OCT 28 2010,1:132

DOI 10.1016/j.jep.2010.07.037 PubMed 20732401

Abstract Ethnopharmacological relevance: The rhizome of Salvia miltiorrhiza Bunge (SM, family Labiatae), which contains tanshinones as main constituents, has been used as a cardiovascular and anti-inflammatory agent in Chinese medicine.Aim of the study: This study aimed to elucidate anti-allergic effects of the root of Salvia miltiorrhiza Bunge (SM, family Labiatae) and its main constituents, tanshinones, against passive cutaneous anaphylaxis (PCA) reaction.Materials and methods: PCA reaction was induced by IgE-antigen complex (LAC) in ICR mice. Protein expression of IL-4 and INF-alpha in rat basophilic leukemia (RBL)-2H3 cells was performed by enzyme-linked immunosorbent assay and NF-kappa B and c-jun (AP-1) activation assayed by immunoblot.Results: Tanshinones inhibited the PCA reaction and reduced IL-4 and TNF-alpha production in mice as well as in IAC-stimulated RBL-2H3 cells. Tanshinones also inhibited NF-kappa B and AP-1 activation in RBL-2H3 cells stimulated with IAC. Among tested tanshinones, tanshinone I exhibited the most potent inhibition, followed by 15,16-dihydrotanshinone I, tanshinone IIA and cryptotanshinone.Conclusions: SM and tanshinones may ameliorate the PCA reaction by inhibiting the allergic cytokines IL-4 and TNF-alpha via NF-kappa B and AP-1 pathways. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

Species covered 丹参 Salvia miltiorrhiza