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The antithrombotic effect of RSNK in blood-stasis model rats

作者 Dang, Xuan; Miao, Jing-jing; Chen, An-qi; Li, Peng; Chen, Lin; Liang, Jin-ru; Xie, Ren-ming; Zhao, Ye

期刊 JOURNAL OF ETHNOPHARMACOLOGY , SEP 15 2015,:173

DOI 10.1016/j.jep.2015.06.030 PubMed 26216512

摘要 Ethnopharmacological relevance: Reduction of Sheng-Nao-Kang decoction (RSNK), composed of Salvia miltiorrhiza Bge., Ligusticum chuanxiong Hort., Astragalus membranaceus (Fisch.) Bunge., Pueraria lobata (Willd.) Ohwi., Paeonia lactifiora Pall. and Panax notoginseng (Burk.) F. H. Chen., is a modified traditional Chinese medicinal formula of Sheng-Nao-Kang pill preparation, which has been investigated its protective effect on focal cerebral ischemia-reperfusion injury in rat in our previous report.Aim of the study: To evaluate the antithrombotic effect of RSNK in blood stasis model rats and explore the potential mechanisms. Materials and methods: Subcutaneous injection of norepinephrine and bovine serum albumin combined with ice water bath was used to establish the acute blood stasis rat model. The anticoagulant activities were investigated by measuring activated partial thromboplastin time (APTT), thrombin time (TT), prothrombin time (PT), and the content of fibrinogen (FIB). Meanwhile, the levels of thromboxane A(2) (TXA(2)), prostaglandins I-2 (PGI(2)), endothelial nitric oxide synthase (eNOS) and endothelin (ET) were detected.Results: The treatment of RSNK was able to prolong APT!'', TT and PT, and decrease FIB content obviously. Furthermore, it markedly suppressed TXB2 level and up-regulated 6-keto-PGF1(alpha) level of the blood-stasis model rats, accompanied with the decrease of T/K. The level of ET and TXA(2) in plasma was down-regulated and the levels of eNOS in plasma and PGI(2) in serum was up-regulated in RSNK-treated rats compared with model rats (P<0.05).Conclusion: The present study suggested that RSNK possessed remarkable antithrombotic property in blood stasis model rats induced by ice water bath and subcutaneous injection of norepinephrine and bovine serum albumin. This property could be associated with its anticoagulation activity, the regulation of active substances in vascular endothelium and maintaining the balance of TXA(2) and PGI(2). (C) (2)015 Elsevier Ireland Ltd. All rights reserved.

涉及物种 丹参 Salvia miltiorrhiza 黄芪 Astragalus membranaceus 芍药属 Paeonia 葛 Pueraria lobata 川芎 Ligusticum chuanxiong