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Simultaneous determination of nine major flavonoids in Sophora flavescens by RP-LC

Authors Ma, Xiao-Chi; Xin, Xiu-Lan; Liu, Ke-Xin; Zhang, Bao-Jing; Li, Feng-Yun; Guo, De-An

Journal CHROMATOGRAPHIA , SEP 2008,5-6:68

DOI 10.1365/s10337-008-0719-y

Abstract A liquid chromatographic method was applied to determine trifolirhizin, kushenol K, kushenol L, kushenol N, kushenol X, kurarinone, norkurarinone, isokurarinone and kushenol A in the roots of Sophora flavescens, namely Kushen in China. The samples were separated on a YMC-C-18 column (250 x 4.6 mm, 5 mu m) with a gradient of methanol and 0.3% aqueous acetic acid (v/v) at a flow rate of 0.8 mL min(-1) and detected at 295 nm. The complete separation was achieved within 45 min for the nine major flavonoids. All calibration curves expressed good linearity (r(2) > 0.999) within the test range. The recovery of this method was 92.3-106.9%. The assay was successfully applied to the quantification of nine flavonoids in 26 samples of Kushen. The results indicated that this developed LC assay could be readily utilized as a quality control method for the Chinese herb medicine Kushen.

Species covered 苦参 Sophora flavescens