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In vitro screening on amyloid precursor protein modulation of plants used in Ayurvedic and Traditional Chinese medicine for memory improvement

作者 Liu, Liang-Feng; Durairajan, Siva Sundara Kumar; Lu, Jia-Hong; Koo, Irene; Li, Min

期刊 JOURNAL OF ETHNOPHARMACOLOGY , JUN 1 2012,2:141

DOI 10.1016/j.jep.2011.08.065 PubMed 21920424

摘要 Ethnopharmacological relevance: The 15 herbs for the screening have been traditionally used in Ayurvedic medicine or in Traditional Chinese medicine (TCM) for the treatment of cognitive disorders clinically.Aim of the study: Fifteen plant species were investigated for the inhibition of amyloid peptide (A beta) production and modulation of amyloid precursor protein (APP) processing.Materials and methods: The selected plants were extracted successively with 70% ethyl alcohol and absolute alcohol concentrated with rotary evaporation then lyophilized. Using a mouse neuroblastoma cells expressing Swedish APP (N2a-SweAPP), MTT assay was performed to determine the toxicity concentration of each herbal extract. In order to evaluate the activity of ethanol extracts on A beta inhibition, the N2a-SweAPP cells were treated with a high and low dosage of different extracts for 24 h, A beta s levels in the supernatant of conditioned media were assessed by ELISA. The most active extracts were then subjected to test the effect on APP modulation in N2a-SweAPP cells by determining their effect on sAPP alpha and 5APP beta through western blot analysis.Results: Among the screened herbal extracts, only Polygonum multiflorum Thunb. (root) and Convolvulus pluricaulis Choisy. (leaves) showed profound inhibition of A beta production. MTT assay demonstrated that the anti-A beta effect of these extracts was not a sequential consequence of their cytotoxicity. The extract of Polygonum multiflorum Thunb. (root) could reduce A beta production only through APP modulation, which was exhibited together with the up-regulation of sAPP alpha and down-regulation of sAPP beta.Conclusion: The results show that extract of Polygon urn multiflorum Thunb. (root) can lower A beta generation by modulating APP processing in the N2a-SwedAPP cell line. These results corroborate the traditional use of Polygonum multiflorum Thunb. (root) for the treatment of cognitive disorders including Alzheimer''s disease (AD). (C) 2011 Elsevier Ireland Ltd. All rights reserved.

涉及物种 何首乌 Fallopia multiflora